二十碳五烯酸的菌种选育及发酵条件
STRAIN SCREENING AND THE FERMENTATTION CONDITIONS OF EICOSAPENTAENOIC ACID
杨革 徐承水
摘 要：利用含亚麻子油的斜面培养基连续传代和逐渐降低培养温度,诱导筛选的方法,从大量丝状真菌中选育到一株产二十碳五烯酸(all-cis-5,8、11、14、17--eicosapentaenoicacid)较高的被孢霉菌(Motierellasp.)SM481.研究得到最适培养基及最适培养条件.在最适培养及产二十碳五烯酸条件下,细胞干重和二十碳五烯酸产量分别为28.8g/L和0.127g/L
关键词：二十碳五烯酸,被孢霉,发酵调控
分类号：Q939.97 文献标识码：A
文章编号：1007-3515(2000)03-0366-0370
作者单位：杨革（山东曲阜师范大学生物系曲阜 273165）
徐承水（山东曲阜师范大学生物系曲阜 273165）
参考文献：
［1］Gellerman J,Scblenk H,1979.Methyl directed desaturation of arachidonic acid to eicosapentaenoic acid in the fungus Saprolegnia parasitica.Biochem Biophy Acta.573:23～30
［2］Jareonkitmongkol S,1993.Production of eicosapentaenoic acid by Mortierella strains.J Am Oil Chem Soc,70(2):199～123
［3］Pigott G M,Tucker B W.,1990.Seafood:effects of technology on nutrition.New York:262～268
［4］Pratima B,Pramod K B,1993.Eicosapentaenoic acid production from microorganisms:a review.J Biotechnology,30:161～183
［5］Sakayu S,Hiroshi K,AkimotoK,et al.,1989.Microbial conversion of an oil containing α-linolenic acid to an oil containing eicosapentaenoic acid.J Am Oil Chem Soc,66(3):342～347
［6］Yong M W,Ward O P,1989.Omega-3 fatty acids:altemative sources of production.Process Biochem,8:117～125
［7］Yong Manitch W,Ward O P,1991.Optimization of procuction of eicosapentaenoic acid by Phaeodtylum tricornutum.Appl Environ Microbiol,57(2):419～425
