朱惠照; 李明焱; 楚桐丽; 魏美芝; 郑化先; 刘楠
浙江寿仙谷生物科技有限公司; 金华寿仙谷药业有限公司
【中文摘要】 通过对选育出的赤芝(Ganoderma lucidum)品种—"仙芝1号"采用拮抗试验、分子生物学实验进行遗传分析,结果表明"仙芝1号"同"仙源5号"及韩芝有明显拮抗效应,其ERIC-PCR扩增图谱与其它品种差异明显,因此选育出的"仙芝1号"是不同于其他灵芝品种的赤芝新品种。
【英文摘要】 Antagonism tests and PCR amplification methodology were used to identify a strain of Ganoderma lucidum designated Xianzhi 1.The original parental strain was isolated from a fruit body collected from the wild and was subjected to subsequent long-term selection procedures.Xianzhi 1 exhibited an antagonistic reaction with a selected intermediate parent strain,Xianyuan 5,and several other G.lucidum cultivars.PCR amplification revealed a band that was specific for G.lucidum,and ERIC-PCR generated an amplification pattern that distinguished Xianzhi 1 from ten other G.lucidum cultivars indicating that it was a new strain.
【中文关键词】 “仙芝1号”赤芝; 拮抗试验; ERIC-PCR
【英文关键词】 Ganoderma lucidum; strain Xianzhi 1; antagonistic reaction; ERIC-PCR
【基金】浙江省重点农业科研项目“精深加工用灵芝品种选育及高效配套栽培技术研究”(编号:2006C22079)的部分研究内容
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,2009年03期 【DOI】CNKI:SUN:SYJB.0.2009-03-006
