沙涛; 丁骅孙
云南大学生物学系!云南昆明650091; 云南大学生物学系!云南昆明650091
【中文摘要】 本文应用改良的滨田氏松茸菌培养法对珍稀濒危的食用菌松茸进行了菌丝体的组织分离及孢子分离 ,获得了松茸组织分离纯培养菌丝体。比较了几种不同成分的培养基的分离效果 ,并对所获菌丝体的形态特征进行了初步研究 ,经 2 6代移种驯化 ,其中 2支松茸组织分离菌丝体性状稳定 ,在改良的PDA培养基上生长良好
【英文摘要】 Isolation of Tricholoma matsutake was performed on several media from the organization and spore by Hamada's method.Filament products were obtained from the organization but no filament was observed due to the failure of germination of spore of T.matsutake.The features of these filaments were characterized. After transferring 26 generation,2 of them grow well on the modified potato dextrose agar medium.
【中文关键词】 松茸; 组织分离; 孢子分离; 纯培养菌丝体
【英文关键词】 Tricholoma matsutake; Isolation; Filament
【文献出处】 中国食用菌,EDIBLE FUNGI OF CHINA,编辑部邮箱,2000年05期 【DOI】CNKI:SUN:ZSYJ.0.2000-05-003
